• Biochemical Assays
• Cellular Assays
• Miscellaneous Assays
• ADME Assays

Quantity of sample required:

• 1 gm sample in a properly closed air tight container. In case the sample is scarce and expensive, lesser quantities can also suffice (Please specify the assays for us to provide the minimum sample quantity requirement).

Turn Around Time:

• The table indicates typical turn around time required for performing the assays. Please enquire about the exact turn around time, at the time of order

Terms and conditions:

• Samples with poor solubility profile in aqueous buffers may not be suitable for analysis.
• IC50 / EC50 values can be calculated only for active samples. For samples which do not have sufficient activity the results may not be amenable to statistical analysis or graph plotting and they will be reported as such. In most cases, IC50 will be calculated using linear regression analysis.
• Details about methodology adopted, importance and relevance of these assays are available on request. For a few assays, positive control data may not be available.
• For all bio-chemical assays, each sample is normally tested at five different concentrations, in duplicate. However for some specific assays a different approach is adopted to obtain reliable results. For ex. the PPAR receptor functional assay is performed in agonist mode using 12 concentrations in triplicate. EC50 is presented only when concentration dependant response is observed. Similarly SIRT-1 assay is performed in activation mode with 5 concentrations in 3-5 replicates and the results are analyzed using a suitable test of hypothesis or represented as fold activation.
• The cost for cell based assays includes determination of the measured parameter at nine pre-determined concentrations, for herbal extracts (0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, 100 �g/ml) and for pure compounds (10-9, 10-8, 10-7, 10-6, 10-5 M), with 4 replicates per concentration. Parallel cell viability estimation is also included in the same cost. These concentrations can be modified as per user request.
• For Ames test, the test substance will be screened at 6 non-cytotoxic concentrations, in triplicate, in the presence and absence of metabolic activator.
• Samples should be preferably free of preservatives for testing in cellular assays. In case preservatives have been added, information should be provided as such samples will require additional testing of preservative controls for obtaining reliable results.
• The results reported by the lab will be applicable to the given test conditions of NRPL assay protocol. It should be noted sometimes results can vary across labs due to variation in assay conditions
• In rare occasions, some assays may be temporarily unavailable due to reagent unavailability. Further new assays are being added regularly, please request for the available list of assays at the time of ordering.